Bloodstream infection (BSI) continues to be a life threatening condition. The host response to such infection varies from clinical signs. In this study, blood samples were collected from immonocompromised patients having leukemia and immunocompetent patients during onset of fever. blood samples were collected at fever onset to predict gram negative bacteremia and study the relation between virulence factors represented as lipase and protease enzymes production in gram negative bacteremia in comparison to antibacterial resistance profiles. Twenty four (24) feverish in-patients were enrolled in the study. Blood samples were collected and cultured on blood culture media for isolation of gram negative organisms and identified by API 20E technique then antimicrobial susceptibility tests and enzymatic activity of lipase and protease were performed.Gram negative bacteria isolated from blood samples showed high resistance against most of the antibacterial agents used. Even in presence of protease and lipase activity as virulence factors.We concluded that continuous monitoring of antimicrobial resistance is required among in-patients to decrease the risk of bacteremia and resistance.