Curdlan is a linear D-glucans, belongs to the class known as beta (1, 3) - D -glucan macromolecules, Curdlan gum is a neutral polysaccharide was discovered by Dr. Harada in 1964, Curdlan gum is insoluble in water but soluble in most organic solvents. Curdlan is one of the FDA approved biopolymer used in food industries such as jelly, noodles, and edible fibers manufacturing process approved in 1996, Curdlan gum produced by many Agrobacterium species under nitrogen-limiting conditions, has many useful benefits in food, agriculture, pharmaceutical, and medicine industry. Sequential optimization strategy, based on statistical experimental designs, was employed to enhance the production of curdlan by local Agrobacterium sp isolate, which include (Plackett–Burman design and Box–Behnken design)