A Series of pyridinium, piperidinium and morpholinium derived cationic surfactants differing in the length of side alkylating chain from C₁₀ to C₁₈ is described. The compounds were characterized by IR and H¹NMR spectra and an HPLC method used for distintion of all prepared long-chain pyridinium, piperidinium and morpholinium analogues has been successfully developed. The surface properties of these surfactants including critical micelle concentration (CMC), efficiency (Pc₂₀), effectiveness (_cmc), maximium surface excess (r_max) minimum surface area (A_min) and free energy of micellization (ΔG°_mic) were investigated. The results of studies on hemolytic and antioxidative activities of the prepared compounds differing in alkyl chain length are presented. The hemolytic studies permitted to determine the safe concentration at which the compounds studied did not damage RBC membranes and they may also be used as effective antioxidants.