Pinocembrin (PIN), a flavanone found abundantly in honey and propolis, has been reported to have many benefits and medicinal properties. However, its protective effects against carbon tetrachloride (CCl₄) induced hepatotoxicity have not been clarified. The aim of the present study was to investigate the potential hepatoprotective dose of PIN on CCl₄ treated rats. PIN was screened at different doses (10, 20 and 40 mg/kg/day) orally for 7 days, against a single dose of CCl₄ (1 ml/kg, 1:1 mixture with corn oil, i.p.). PIN protected against CCl₄-induced increase in hepatic transaminases, total cholesterol and histopathological changes. The dose of 20 mg/kg PIN was selected for further assessment to address the PIN hepatoprotective mechanisms.