There has been some evidence to date suggesting that Trichoderma species. had several biological activities. To test antibacterial and antifungal activity, the secondary metabolites obtained by culturing the fungi Trichoderma viride RCMB 017002 on YES broth for 21 days were extracted using chloroform/methanol solvent system then screened by agar well diffusion method. Two fungal metabolites were isolated and purified from the chloroform/methanol (2:1) extract of Trichoderma viride broth culture using column chromatography. The chemical structures of the separated metabolites were elucidated using the spectroscopic methods FTIR, ¹HNMR and EI-MS analyses. The first compound was benzene diamine derivative produced from fraction 10 and showed good inhibitory activity against the tested bacteria, and tumor (against HepG2 & MCF-7) cells. Also, compound 1 showed moderate antiviral activity against herpes simplex type 1 virus along with no antifungal activities. However, compound 2 was with the known compound, 6-pentyl-α-pyrone that produced from fraction 17
 with broad spectrum antimicrobial activity as well as moderate antitumor activities and weak antiviral activity.This report demonstrates the biological activities of new fungal metabolites from Trichoderma viride as potential drug that need further studies and development.