In the present work, the shrub W. somnifera was used as a source for the extraction of bioactive molecules. Leaves were extracted by ethanol and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) and High-Performance Liquid Chromatography (HPLC). By GC-MS analysis, the highest identified compounds were 2,6-dimethyl-N-(2-methyl-α-phenylbenzyl)aniline, 4-Aminoheptane, n-undecanophenone, 2-cyclohexyl-4-hydroxymethyl-octahydrobenzo[e][1,2] oxazine-3-carbonitrile, and octadecanoic acid 3-octadecyloxypropyl ester, with percentages of 19.43%, 7.51%, 4.47%, 4.34%, and 3.89%, respectively. HPLC analysis identified quercetin and 7-hydroxyflavone with concentrations of 12.36 and 15.14 mg/mL, respectively, as two flavonoid compounds. This work showed that W. somnifera leaf extract owns several bioactive compounds that could be useful and have different applications, such as antimicrobial activities.