This experiment was conducted at the Nursery of the Department of Floriculture, Ornamental
Horticulture and landscape Gardening - Faculty of Agriculture Shatby - University of Alexandria in two
successive seasons (2014-2015 and 2015-2016), to study the biological treatment of contaminated soil with
some heavy metals using some trees, namely;
1. Acacia saligna (inoculated with Rhizobium sp.,).
2. Casuarina glauca: which inoculated with Frankia.
The treatments of the heavy metals (Cd - Zn - Pb) were: Control - 250 - 500 and 1000 mg/kg soil. The
most important results follows:
 The application of the high level of heavy metal (500- 1000 ppm) lead to decrease the growth parameters in
both seedlings. The negative effect of the pollutant was more significant in uninoculated seedlings with
Rhizobium and Frankia.
 Both of Cd and Pb had harmful impact on plant growth than that the Zn did. On the other hand, the effect of
the Zn content was more harmful on C. glauca seedlings.
 Chlorophyll contents of the phyllodes were decreased as a result of application of heavy metal.
 The inoculated plants with Rhizobium and Frankia had displayed a significant increase in carotine content
under high levels of heavy metals as it compared with uninoculated ones.
 Nitrogen and phosphorus contents were decreased in the phyllodes of inoculated seedlings with Rhizobium
and branchlets of Frankia with increasing of heavy metal level.
 The concentration of lead in the phyllodes was higher than that detected in the roots, particularly in
uninoculated seedlings with Rhizobium, while for the Casuarina seedlings, both of Pb and Zn in the roots
was higher than that in the leaves.
Advisors Committee Examiners Committee
Prof. Dr. Tarek Mahmoud El-kiey Prof. Dr. Emam Mohamed Saber Nofal
Prof. Dr. Ahmed A. A. El-Settawy Prof. Dr. Essam Abd El-Rahman Koreish