C-band heteromorphisms including intra-pair asymmetry and inversions as well as deviation in size have been reported to be associated with some but not all human malignancies. The study was conducted with the aim of investigating intra-pair asymmetry, inversions and deviation in size of the C-band region of chromosomes 1, 9 and 16 in patients with lung cancer as compared to a group of healthy subjects. The study included 39 male patients with lung cancer and 29 normal healthy male subjects as a control. The size heteromorphism was evaluated using 16p as a reference. Heteromorphism between homologous chromosomes was considered when there was an intra-pair difference of one or more levels. Inversions whether partial [more than 1/4th of the C-band located on the short arm] or total were recorded. There was no significant difference in heterochromatin size between patients and control. Comparing the symmetry and heteromorphic pattern in chromosomes 1,9,16 revealed that heteromorphism was more frequent in lung cancer patients compared to the control groups; 56.41% [22/39] of patients with lung cancer showed heteromorphism in chromosome No 1 compared to 37.93% [11/29] in the control group, but the difference was statistically not significant [X2 = 2.3]. Heteromorphism of chromosome No 9 was present in 51.28% [20/39] in lung cancer patients as compared to 34.48% [10/29] of the control, the difference was statistically not significant [X2 = 2]. Heteromorphism of chromosome No 16 was present in 23.08% [9/39] of lung cancer patients as compared to 13.79% [4/29] of the control group, but the difference was statistically not significant [X2 = 0.97]. The frequency of localization variants was higher in the lung cancer patients than the control; in patients with lung cancer, the frequency of inversions in chromosome No 1 was 8.98% [7/ 78] compared to 5.18% [3/58] in the control group. The frequency of inversions in chromosome 9 was 5.13% [4/78] in the group with lung cancer as compared to 1.73% [1/58] in the control group. The differences were statistically not significant.