The herein study was endeavored to evaluate the influence of low level laser therapy [LLLT] as an antioxidant on alcohol induced stressors in rats through the determination of malondialdeyde [MDA], glutathione content [GSH], and glutathione peroxidase [GPx] in the blood of 40 rats divided into 4 groups; 10 rats as a control group, 10 rats which ingested ethanol, 10 rats exposed to LLLT, and 10 rats which ingested ethanol and exposed to LLLT. The data of this study revealed that during chronic ethanol consumption there was a high significant increase in the level of MDA associated with a significant decrease in the levels of both blood GSH content as well as the erythrocyte GPx enzyme activity. After applying laser to rats which ingested alcohol there was a significant decrease in the level of MDA associated with a high significant increase in the enzymatic activity of GPx and a significant increase in the GSH content when compared to rats which ingested alcohol alone.
Conclusively, the results of the present study revealed that LLLT have the potential for stimulating enzyme activities which might contribute to increase glutathione concentration and provide protection against oxidative damage. Furthermore, LLLT resulted in a significant increase in the main enzyme of antioxidant system which was glutathione peroxidase resulting in a significant reduction in lipid peroxidation intensity [decrease in the level of serum content of malondialdehyde]. These data suggest that LLLT might have an antioxidant properties and this could explain its therapeutic effects in some pathological conditions.