A Simple method was used for the determination of patulin in apple juice. The samples were extracted with ethyl acetate and the extract was cleaned up by extracting with sodium carbonate solution. Patulin was then determined by reverse phase high pressure liquid chromatography [HPLC] using a 25 m Zorbax XDB C18 column and a variable wavelength detector set at 276 nm. Water-Acetonitrile [4:96] were used as the mobile phase at a flow rate of 1 ml/min. The occurrence of patulin as a toxic mycotoxin was investigated in 120 samples of apple Juice commercially available in Saudi Arabia by using an HPLC method with a concentrations range from 0.057 to 0.104 μg/ml. The primary aim of this investigation was to determine the amount of patulin content in apple juice as it may cause health problems.