The present study has been planned to evaluate the effect of alcohol and bhang on some biochemical parameters in human and their relation with health. The present results indicated that alcohol produced a significant increase in serum α-glutathione-S-transferase [α-GST]. Alcohol and bhang increased serum gamma glutamyl transferase [y-GT], alanine transaminase [ALT], aspartate transaminase [AST], alkaline phosphatase [ALP], creatine kinase [CK], and creatinine levels in comparison with controls. These results showed the harmful effects of alcohol and bhang on health. However, the present work along with other findings in the literature suggest the fact that although the alcohol and bhang absusers may superficially look health, biochemical tests have indicated early sings of health deterioration.