The effect of electromagnetic field (EMF) on serum thyroid stimulating hormone (TSH) and triiodothyronine-thyroxin (T3-T4) hormones levels of adult male Sprague-Dawley rats were studied. Fifty rats were used in three independent groups, 20 of which were control (without stress and EMF), 20 of which were exposed to 900 MHz EMF and 10 of which were sham-exposed. The exposures were performed 30 min/day, for 5 days/week for 4 weeks to 900 MHz EMF. Sham-exposed animals were kept under the same environmental conditions as the study groups except with no EMF exposure. The concentration of TSH and T3-T4 hormones in the rat serum was measured by using an enzyme liked immunsorbent assay (ELISA) method for TS, T3, and T4 hormones. TSH values and T3-T4 at the 900 MHz EMF group were significantly lower than the sham-exposed group (p<0.01). There were no statistically significant differences in serum TSH values and T3-T4 hormones concentrations between the control and the sham-exposed group (p>0.05). These results indicate that 900 MHz EMF emitted by cellular telephones decrease serum TSH and T3-T4 levels.