Reproductive health is a crucial part of the general health, not only as a key element of the health during adolescence and adulthood but also through sitting the stage for health beyond the reproductive years for both women and men. The aim of the study is to evaluate  the effect of  enhancement of knowledge and attitude of secondary school girls regarding reproductive health. The present study hypothized  that most of girls had lack of knowledge and misconception about reproductive health issues  which may negatively affect their attitude before providing them with guideline. The study conducted at Alshiamaa secondary school located in Benha City at Kaluobia Governorate The sample included 160 girls in adolescent period, from third year. The tools of data collection were structured demographic sheet, knowledge questionnaire sheet and modified Likert Scale. The result showed that 58.8% of secondary school girls who are included in the study had poor knowledge about reproductive health issues and 67.5% of them had negative attitude about it.  The study recommended that, developing reproductive health awareness programs targeted to adolescents on large sample size, in different schools to high light this issue.