20868

Comparison of Standard Methods, MTF, and MF, by the Colilert Rapid Test for the Enumeration of Coliform Bacteria in High Salinity Water, Arabian Gulf, Doha, Qatar

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Last updated: 03 Jan 2025

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Abstract

Colilert Rapid test was compared with the traditional methods (multiple tube fermentation (MTF) and Membrane filtration (MF)) to enumerate total and fecal coliform in marine water. Colilert method is based on specific indicator nutrients: ONPG and MUG for the target microbes, and chemically suppresses non-coliform bacterial growth. It is more rapid, providing results in as little as 18 h compared to the 24–96 h required for traditional methods. The results reported that Colilert method was more sensitive in detecting fecal coliform than the traditional methods (MTF and MF). No significance difference between it and MF in enumeration of total coliform. While, there were significant differences with both MF and MTF in enumeration of fecal coliform. It was more close to MF than MTF. The study recommended MF technique for detecting and enumerating coliform bacteria in marine water due to the high values of false positive results detected by Colilert method.

DOI

10.21608/jhiph.2008.20868

Keywords

MTF, and MF, Colilert Rapid test, Coliform bacteria, High Salinity Water, Arabian Gulf, Doha, Qatar

Authors

First Name

Magda

Last Name

EL-Magharaby

MiddleName

Abed El-Atty

Affiliation

Department of Environmental Health (Division of Environmental Chemistry) and Biology, High Institute of Public Health), Alexandria University, Alexandria, Egypt

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Volume

38

Article Issue

1

Related Issue

4137

Issue Date

2008-01-01

Receive Date

2018-12-07

Publish Date

2008-01-01

Page Start

77

Page End

90

Print ISSN

2357-0601

Online ISSN

2357-061X

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https://jhiphalexu.journals.ekb.eg/article_20868.html

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https://jhiphalexu.journals.ekb.eg/service?article_code=20868

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4

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Original Article

Type Code

511

Publication Type

Journal

Publication Title

Journal of High Institute of Public Health

Publication Link

https://jhiphalexu.journals.ekb.eg/

MainTitle

Comparison of Standard Methods, MTF, and MF, by the Colilert Rapid Test for the Enumeration of Coliform Bacteria in High Salinity Water, Arabian Gulf, Doha, Qatar

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Article

Created At

22 Jan 2023