Background: Diarrhoea is one of the leading causes of morbidity and mortality in children worldwide. Faecal screening methods as detection of faecal leucocytes, faecal lactoferrin and faecal occult blood, have diagnostic and therapeutic implications in the provisional diagnosis of invasive diarrhea before culture results made available. Objective: The aim of this work was to outline the bacterial and parasitic profile of acute pediatric diarrhoea and to evaluate faecal screening tests in preliminary diagnosis of invasive diarrhoea. Methods: Three hundred children under five years of age, suffering from acute diarrhoea (Results: Forty eight percent of samples were positive for enteric pathogens. Enteric bacterial pathogens were isolated from 25% of samples. Parasites 29% and mixed bacterial and parasitic infections were detected in 6% of samples.  Enterotoxigenic E.coli (ETEC) was the most common bacterial isolate detected in10% of samples, followed by Salmonella (8%), Shigella (6.67%) Campylobacter (5%) and Vibrio parahaemolyticus (1.33%). Cryptosporidium was the most commonly identified parasite (13%) followed by Giardi lamblia (11%), Entamoeba histolytica (8%) and Cyclospora cayetanensis (3%). Ascaris lumbricoides and Haeminolipus nana were only identified in 1% of samples, each. The gold standard for evaluation of faecal screening tests was positive culture for invasive bacterial pathogens and/or positive E. histolytica on microscopic examination of stool samples. Leuko test had the highest sensitivity (85.54%), specificity (73.73%), positive predictive value (55.47%), negative predictive value (93.02%) and accuracy (77%). False positive results of the Leuko-test were significantly higher in the breast-fed children than non breast-fed ones (26.7%, 11.7% respectively, p < 0.01). Better sensitivity, specificity, positive and negative predictive values of the Leuko-test was recorded in the non breast-fed children than in the breast-fed ones. The recorded values in the first group were: 91.11%.83.64%, 69.5% and 95.83%, respectively compared to 78.95%, 63.55%, 43.48% and 89.47%, respectively in the second group. Conclusion: The study concluded that, Leuko test is the best applicable faecal screening test in differentiation of invasive and non invasive diarrhoea but is better avoided in breast-fed infants as many false positive results might be interpreted.