Background: Undergraduate medical education in many developing nations is facing new challenges today and the performances of students as well as physicians in the community are perceived to have largely declined. Objectives: to determine the association between life; study habits and the final year grades of the medical students in Mansoura University. Methods: This was a Comparative Cross-Sectional Study. It was carried out from May to August 2013 among the total of 802 students, who were registered in the practical course that follows their latest years. The data of which was collected using a self-administrated questionnaire. The questionnaire included four parts; the first part covered the personal data. The second part of the questionnaire included the life-style of medical students, and the third part included the studying habits of medical students. Results: 30.3% of students were ranked as excellent according to latest year grades (2012- 2013). The most important life-style significant factors affecting the final year grades were internet use and sleeping hours, and the most significant study habits' factors were English proficiency, and study motivation. Conclusion: In this study internet using, sleeping hours, English proficiency, attending tutorials and study motives are the most related factors to the study performance of medical students.