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276219

Phenotypic and genotypic characterizations of antimicrobial resistance among gram-negative bacilli of clinical isolates

Article

Last updated: 23 Dec 2024

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Subjects

-

Tags

Human Microbial Interactions
Medical Microbiology

Abstract

One of the biggest threats to human health today is the emergence of resistance among the most significant bacterial diseases. To identify outbreaks and the transmission of clinically important resistance genes and the genetic components associated with them in human infections, there is a need for genomic surveillance of antimicrobial resistance genes. The objective of this study is to evaluate the phenotype and genotype of antimicrobial resistance in clinically isolated gram-negative microorganism strains, as well as their molecular characterization. Microbiological identification was done using the automatic microbiological analyzer and GN ID REF21341 cards. The isolates' susceptibility to antimicrobials was evaluated using conventional disc diffusion. Using particular primers, resistance genes were amplified through PCR (applied biosystems).The identities of all 1470 isolates, including Escherichia coli (28%), Klebsiella pneumoniae (21%), Acinetobactor baumanii (7%), Serratia marcescens (6%), Enterobacter cloacae (6%), Proteus mirabilis (6%), Pseudomonas aeruginosa (5%), Citrobacter freundii (5%), Citrobacter braakii, Acinetobacter lwoffi, Enterobacter aerogenes and Proteus vulgaris (4%). All of these isolates exhibited multidrug resistance (MDR) to several kinds of antimicrobial medicines. Nine Carbapenem-resistant gram-negative bacilli strains were identified to be positive for blaNDM and blaOXA-1.Our study found a concerning association between these diseases' antimicrobial resistance and the routinely prescribed antibiotics. This discovery compromises the medical field's therapeutic options and encourages the use of specialists who have less potent antimicrobial effects.

DOI

10.21608/mb.2022.153994.1062

Keywords

Antimicrobial-resistance, Gram-negative bacteria, carbapenemases, genotype

Authors

First Name

Mukesh

Last Name

Sharma

MiddleName

Kumar

Affiliation

Department of Biotechnology, Maharaj Vinayak Global University, Jaipur, India.

Email

mukeshsharma.dt@gmail.com

City

Jaipur

Orcid

0000-0002-4668-9531

First Name

Moh. Rizvan

Last Name

Qurashi

MiddleName

-

Affiliation

Department of Botany, Vishwa Bharti PG College, Sikar, India.

Email

mohrizvanmedmicro777@gmail.com

City

Jaipur

Orcid

-

First Name

Sunil

Last Name

Sharma

MiddleName

Kumar

Affiliation

Department of Botany, Jai Narain Vyas University, Jodhpur, India.

Email

sunilbhardwaj508@gmail.com

City

Jodhpur

Orcid

-

Volume

7

Article Issue

2

Related Issue

38239

Issue Date

2022-12-01

Receive Date

2022-08-03

Publish Date

2022-12-26

Page Start

1

Page End

8

Print ISSN

2357-0326

Online ISSN

2357-0334

Article File

MB_Volume 7_Issue 2_Pages 1-8.pdf

PDF . 458.5kB

Link

https://mb.journals.ekb.eg/article_276219.html

Detail API

https://mb.journals.ekb.eg/service?article_code=276219

Order

2

Type

Original Article

Type Code

502

Publication Type

Journal

Publication Title

Microbial Biosystems

Publication Link

https://mb.journals.ekb.eg/

MainTitle

Phenotypic and genotypic characterizations of antimicrobial resistance among gram-negative bacilli of clinical isolates

Details

Type

Article

Created At

22 Jan 2023