Saliva is thought to have a significant impact on the colonization of microorganisms in the oral cavity, the present study aimed to determine and confirming molecularly presence of the pathogenic saliva-bacterium, in addition to elaborating the effect of triclosan washing material on them. The study includes 42 saliva samples which were collected from patients in a private dental clinic from the area facing the lower front teeth. The samples were diagnosed by culture, microscopic and biochemical tests. Microbiologically 20 Klebsiella pneumonia, 16 Pseudomonas aeruginosa and 6 Escherichia coli isolates confirmed. The sensitivity to the triclosan were varies and P. aeruginosa isolates were 80% and followed by 70% and 50% for K. pneumonia and E. coli respectively. The results of the PCR reaction showed that K. pneumonia bacteria with a size of 130 bp, P. aeruginosa bacteria with a size of 500 bp and E. coli with a size of 147 bp. Nitrogenous base sequences results for 6 bacterial isolates were compared with similar standard strains from the Gene bank available on the NCBI website and showed a 100% similarity between the bacterial isolates and those recorded in the gene bank.