ManageChatDiscover
203063

Virulence traits-based behavior of Streptococcus mutans bacteria from dental plaque and dental caries conditions

Article

Last updated: 03 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Behavioral ecology
Human Microbial Interactions
Medical Microbiology
Microbial Ecology

Abstract

Streptococcus mutans has significant virulence factors associated with the etiology and pathophysiology of dental plaque and caries through adherence to the tooth surface and biofilm formation. These microbes can tolerate and survive the acidic environment. The study aimed to compare the behavior of the S. mutans isolates from dental plaque and caries conditions, in addition to the evaluation of the biofilm formation by S. mutans with oral microbiota (Candida albicans). A total of 106 S. mutans isolates were recovered including 56 isolates from soft caries lesions, 30 isolates from the plaque of caries active and 20 isolates from the plaque of caries-free samples. The isolates' virulence efficacy was assessed phenotypically by acid production, acid tolerance, and biofilm formation assays. PCR detected genes encoding these virulence traits. There was a significant difference (p < /em> < 0.05) between the three groups of isolates in biofilm formation, biofilm formation when co-cultivated with C. albicans, pH reduction by acid production, but there was no significant difference in withstanding acidic pH. S. mutans isolates harbored the genes glucosyltransferase B (gtfB), glucan binding protein B (gbpB), F1F0 membrane-bound proton-translocating ATPase C subunit (atpH) and lactate dehydrogenase (ldh) which are responsible for biofilm formation, sucrose-dependent adhesion, acid tolerance and acid production, respectively. In conclusion, S. mutans isolates from caries-free and caries active dental plaque and dental caries conditions showed different virulence efficacy-based behaviour. C. albicans enhances the biofilm formation by S. mutans. Further investigation of factors influencing variable behavior of S. mutans bacteria from different lesions is warranted.

DOI

10.21608/mb.2021.100068.1044

Keywords

Streptococcus mutans, Biofilm, plaque, Virulence factors, dental caries

Authors

First Name

Rana

Last Name

Abo Bakr

MiddleName

A.

Affiliation

Department of Microbiology and Immunology, Faculty of Dentistry, October University for Modern Sciences and Arts (MSA), 6th October City, Giza, Egypt

Email

rbakr@msa.edu.eg

City

Cairo

Orcid

-

First Name

Mahmoud

Last Name

Tawfick

MiddleName

M.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt.

Email

mahmoud_tawfick@azhar.edu.eg

City

Cairo

Orcid

0000-0002-9825-2198

First Name

Zeinab

Last Name

Mostafa

MiddleName

A.

Affiliation

Department of Medical Microbiology, Faculty of Medicine, Cairo University, Cairo, Egypt.

Email

zakhalek@msa.edu.eg

City

Cairo

Orcid

-

First Name

Abeer

Last Name

Abdulall

MiddleName

K.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

Email

profabeerkhairy2020@gmail.com

City

Cairo

Orcid

0000-0002-0750-6878

Volume

6

Article Issue

1

Related Issue

24927

Issue Date

2021-06-01

Receive Date

2021-10-08

Publish Date

2021-11-04

Page Start

75

Page End

85

Print ISSN

2357-0326

Online ISSN

2357-0334

Article File

MB_Volume 6_Issue 1_Pages 75-85.pdf

PDF . 371.6kB

Link

https://mb.journals.ekb.eg/article_203063.html

Detail API

https://mb.journals.ekb.eg/service?article_code=203063

Order

9

Type

Original Article

Type Code

502

Publication Type

Journal

Publication Title

Microbial Biosystems

Publication Link

https://mb.journals.ekb.eg/

MainTitle

Virulence traits-based behavior of Streptococcus mutans bacteria from dental plaque and dental caries conditions

Details

Type

Article

Created At

22 Jan 2023