Diffuse large B cell lymphoma (DLBCL) is themost predominant type ofNon Hodgkin- Lymphomas,representingmore than one thirdof all recently diagnosedcases.Long non-coding RNAs (lncRNAs) are a class of RNAnot coding for proteinhaving more than 200 nucleotides and have significant roles in tumor formationand they have been investigated in different types of solid and hematologic tumors, including DLBCL.This work aims to studythe level of lncRNAs(NAALADL2 and XIST)gene expression in DLBCL.NAALADL2 and XIST geneexpression levelsin blood were performed by real time qPCR technique.Forty five DLBCL patients and forty five age and gender matched healthy controls were included in this study.There was significant statistical increase in the expression of NAALADL2 and decrease of XIST expressionin patients compared to control group (p value < 0.001). From this study, ROC curve analysis revealed cut-off values>10.3 and<1.57 for NAALADL2 and XISTrespectively.There was significant positive correlation between NAALADL2 and advanced stage and a significant negative correlation is found between XIST and advanced stage.It may be concluded that NAALADL2 and XISTexpression levels were obviously associated with DLBCL and might determine the disease progression.