This investigation was carried out at Agricultural Researches Center Al Giza, Egypt, under greenhouse experiments during 2019 and 2020 winter seasons. The study included the effect of three organic fertilization sources: compost, poultry, vermicompost and without organic fertilizer, with mixed inocula (N₂ fixers Azotobacter chroococcum (A C), phosphate dissolving bacteria (Bacillus megatherium var. phosphaticum (B M), potassium mobilizers (Bacillus circulans (B C)) and without microbial inoculation on height, weight of plant and spike weight as well as (N, P and K) uptake of barley plant variety Giza 126. This experiment was conducted in pots 3 kg. Obtained results showed that the plant height, plant weight and spike weight as well as (N, P and K) uptake of barley plant were increased significantly by adding the poultry or vermicompost compared to without organic fertilization addition in both seasons. Generally, in most cases, all parameters were increased significantly by microbial inoculation compared to the control treatment (without microbial inoculation) in both seasons. The parameters were improved significantly by adding poultry or vermicompost with mixed inocula (N₂ fixers (Azotobacter chroococcum (A C), phosphate dissolving bacteria (Bacillus megatherium var. phosphaticum (B M), potassium mobilizers (Bacillus circulans (B C)) in both seasons. While, the lowest values were recorded by without organic fertilization and microbial inoculation in the both seasons.