River Nile is the most important and principal source of fresh water for Egyptians. Rosetta branch supplies fresh water for the governorates of Western Delta (Giza, Qaliubia, Menofia, Kafr El-Sheikh, Beheira and Alexandria). Rosetta branch receives organic, in-organic pollution and heavy metals through the discharge of municipal sewage and industrial effluents in Elrahawy drain water which is pumped to Rosetta branch. The aim of this work is to investigate pollution loads in Rosetta branch in the path (212-230km); a CaseStudy (Edku-Rosetta) with special reference to chemical parameters. Water samples were collected monthly during the period from June 2015 to May 2016. Samples from water intakes from Edfina and El-Gedia water drinking plant were collected.  Results showed that COD and ammonia recorded 22.0-38.0 ppm and 2.5-4.0 ppm respectively. Also, total nitrogen varied from 5.1-7.1 ppm. The results were out of the permissible levels according to the Egyptian Ministry of Health and the International Standards. It can be concluded that Rosetta branch suffers from pollution loads that should be investigated periodically and controlled.