Fanconi anemia is a genetically and phenotypically heterogenous disorder characterized by DNA repair deficiency and telomeric erosion. Different antioxidants have been assessed to regain the chromosomal stability in Fanconi anemia, among those is the Resveratrol. In Vitro Micronucleus assay provides a convenient and reliable index of both chromosome breakage and chromosome loss assessment. This study aims to assess the effect of Resveratrol on the stability of the chromosomes in Fanconi anemia patients using in vitro micronucleus assay as a parameter of improvement. The present study was conducted on 10 patients and 10 normal controls matching in age and sex. Induction of breakage by 1,3-Butadiene diepoxide and evaluation of the antioxidant effect of Resveratrol on genomic stability using In Vitro Micronucleus assay technique were done. Significant reduction in the level of micronuclei in cultures treated with Resveratrol was found. Resveratrol could be an effective adjuvant therapy for patients with FA to improve their hematological condition, chromosomal stability and it might protect from cancer development.