The experiment was carried out in the experimental farm of El-KanaterResearch Station, Horticulture Research Institute, Kalubia Governorate, Egypt. Cultivation of seedling carried out in two seasons 2011-2012/2012-2013to study the effect ofN.P.K. fertilizers and boifertilizer on Majorana Plant. This work was designed in order to investigate the possibility of reducing the need for NPK chemical fertilization as well as improving plant productivity by using bacterial biofertilization as an alternative. The plants of control treatment received only the full recommended rate of NPK chemical fertilization as ammonium sulphate, calcium superphosphate and potassium sulphate. For the other treatments, a single inoculum or mixture of different inoculum combinations of Azotobacterchroococcum and Azospirillumlipoferum were used in combination with NPK chemical fertilizer as quarter or half dose of the full recommended rate. The experiment was complete random parts to study the effect of nitrogen fixing bacteria application (Azospirillumlipoferum and Azotobacterchroococcum). The results revealed that the application of biological fertilizers plays a remarkable role in improving yield quality and quantity in Marjoram and can be viewed as a suitable replacement for chemical fertilizers. Keywords: Chemical fertilization, Bio-fertilization, Majoranahortensis