Depression is a mood disorder whose main clinic characteristics include lasting low motion, decrease in motivate ability and slowness in function of thinking and recognizing .It is showed in national survey that the suicide rate of people suffering from depression is 20 times higher than that of normal people. The present study aimed to select the most suitable dose of reserpine for induction of depression in mice with minimal side effects and minimal mortality rate. Depression was induced in mice by a single i.p. reserpine injection at different dose (0.1, 0.5 and 1 mg/kg) at the day 1, then forced swimming test was performed 1 hour later at the first day. The duration of the experiment was 2 days. The mortality rate was recorded, The brain was excised and prepared  for determination of the content of the Adenosine monophosphate (AMP), adenosine Diphosphate (ADP) and Adenosine triphosphate (ATP), Monoamine neurotransmitters (5-HT, DA and NE), Malondialdehyde (MDA), Nitric oxide (NO), Glutathione reduced (GSH), oxidized (GSSG) and 8- hydroxyl-2-deoxyguanosine (8-OHDG). The forced swimming test and number of jumping showed no significant difference between different doses while the mortality rate was markedly variable. The results concluded that the most suitable reserpine dose for inducing depression with minimal mortality was 0.1 mg/kg of body weight.