This study purpose is to measure the impact of six sigma methodology on quality of the work life, applying it on the employees in Ain Shams University Hospitals; and identifying as well the importance and mechanisms of applying such methodology in addition to recruiting an applied study for identifying the appropriateness, potentialities and demands of application on Ain Shams University Hospitals the suggested for this study. For achieving this study aims, a questionnaire form is designed, distributed and collected through interview and included a set of phrases for measuring the study sample attitudes towards the study variables. The researchers count on a mixture between the deductive and the inductive method through the theoretical and the field study. Researchers have administered a data analysis employing Kruskal Test for each question in the investigation form for testing validity of the study hypotheses. The study sample consists of (200) items which is the number of the correct retrieved forms. Researchers come to several results and recommendations concerning the study sample (Ain Shams University Hospitals. The study comes to that the application of six sigma methodology on Ain Shams University's Hospitals leads to improvement of work quality of life. The study recommends the following: 1-   The importance of the six sigma methodology in improving quality and reducing costs of quality of work life in increasing the ability of the competitive ability of hospitals that support in turn the costs of the work life quality. 2-   Improving the circumstances of the work environment which supports in turn the role of the six sigma methodology for improving quality and reduce costs and so the work quality.