This work aimed to achieve effective use of some environmental friendly treatments of bio agents (Streptomyces lydicus- Trichodermaharziunum- Trichodermaviride) on specific and technological quality of wheat. This study was carried out in the Abu Rawash province area, Giza Governorate during two successive seasons of 2014/2015 and 2015/2016.Two cultivars grain (Wheat Egypt 1, Wheat Egypt2) were used in this study. The maximum of 1000 grain fresh weight obtained in Streptomyces lydicus followed Trichodermaharziunum and Trichodermaviride in both seasons. While, the lowest value of 1000grain fresh weight were recorded in case of control application. Also, CV. Egypt 1 gave higher fresh weight/ 1000 grain weight, starch and wet- gluten high than CV. Egypt 2 in most cases in both seasons. Streptomyces lydicus treatment in wheat Egypt I recorded highest significant increased wet -gluten (33.33 and 33.60) followed by other treatments. While recorded treatment Streptomyces lydicus with Egypt 2 as compared with the control produced the lowest value with wheat Egypt 2 (27.36 and 27.13) in both seasons. Data showed that there is non-significant difference between Egypt 1 and Egypt 2 cultivars in water absorption in treatments Streptomyces lydicus, methyljasmonate and Trichodermaharziunum, while there is a significant increase in Egypt 2 compared with Egypt 1 cultivars in treatments with Trichodermaviride and control plants. Also, there is non-significant difference between Egypt 1 and Egypt 2 cultivars in arrival time and dough development time in treatments of Streptomyces lydicus, methyljasmonate, Trichodermaharziunum and control except treatment Trichodermaviride for rheological quality.