Biodegradation of Lignocellulosic wastes (cotton stalks and corn cobs) was carried out by single and mixed cultures of Trichoderma viride T3 and three strains of Aspergillus niger with code A2, A4 and An2. Results indicated that biodegradation of cotton stalks and corn cobs by mixed culture gave higher activities for cellulase production and saccarification than single cultures. Mixed culture of Trichoderma viride T3 and Aspergillus niger A4 gave the highest activity of cellulase being 1.02 & 0.94 U/ml with 49.7 & 45.8 % of saccarification on media containing alkaline corn cobs and cotton stalks, respectively. It was observed that the ability of the mixed for degrading corn cobs was more efficient than degrading cotton stalks which increased about 1.08 fold of cellulase activity and saccharification. The biological and enzyme parameters of co-culture on alkaline corn cobs were calculated, which recorded 56.73 % of yield factor, 85.59 % of sugar utilization efficiency, 16.60 % of effective yield, 32.55 % enzyme yield, 37.60 % conversion coefficient with productivity of 0.15 U/ml/d.