The objective of this study was to record baseline data for the Sliver Sabahia chicken strain and evaluate the effect of sex on the various hemato-biochemical parameters. Total of 40 birds at 33 weeks of age (20 male and 20 female) were used. The hematological profile included hemoglobin (Hb), red blood cells (RBCs), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and white blood cells (WBCs), whereas the biochemical parameters include total protein, globulin, cholesterol, uric acid “etc." Significantly (p≤0.05) higher, RBC, PCV, and WBC values were recorded in males than females. Total protein, globulin, cholesterol, triglycerides, calcium, uric acid, alkaline phosphatase were significantly (p < 0.05) higher in females. The rest of the parameters were not significantly different between the sexes. Interestingly, almost all investigated hematological and blood chemical parameters in these chickens were within range of the recorded reference values of healthy birds. This study concluded a significant effect of sex on various physiological characteristics of the Sliver Sabahia chicken strain. Also, this paper exclusively provided reference values of hematological and blood chemistry for this strain, which help in the breeding programs to produce the commercial egg type strain.