The study aims to evaluate genetically milk production of a synthetic maternal line of rabbits (V line) and semen quality of bucks. A total of 77, 70 and 70 does were used to estimate milk yield from kindling up to weaning for more than 4 parities. Twenty males of rabbits at 63 days of age were selected random and blood samples were collected to determine serum follicle-stimulating (FSH), luteinizing (LH) and testosterone hormones at the ages of 63 days and 5 and 6 months. Milk yield were 113.06, 172.87 and 211.76 g at 7th, 14th and 21st days of age after parturition, respectively. It is noticeable that the effect of the month had no significant effect on milk yield at 7th and 21th days of lactation period, while it was affected significantly at 14th days, and the month of December was the highest rate of milk yield in the months of the productive year. The effect of parity number more than 4th on milk yield was increased significantly with the advancement and decreased for other parities. The correlations between FSH, LH and testosterone hormonal levels and rabbits sperm concentration and advance sperm motility were significantly affect at 63 days. But, the correlation between FSH and LH levels and semen volume and total sperm out percentages were significant at 63 days. It was concluded that the semen quality was associated with an increase in testosterone concentrations at 5 and 6 months. In summary, it can be observed that the evaluation of the V line rabbit as a maternal line with high milk yield during the lactation period and that high semen quality for males V line rabbits can be predicted by high levels of hormones LH, FSH and testosterone concentrations at 63 days of age.