The present study was aimed to investigate isolation, cloning and se-quencing of chicken Growth hormone gene (cGH) from chicken (cobb 500 broilers) then transfer chicken Growth hormone gene by two methods the first gene transfer method by using sperm-mediated gene transfer (SMGT) technique and the second gene transfer method using bioresonance (Bio) to local chicken strain (Bandarah).This study was investigate for two generation. The body weight at hatch and 4 weeks of age in-creased by 3.32 and 8.09 g for SMGT method and by 1.27 and 5.22g for Bio method respectively. The SMGT method was increased the body weight at 12 wk of age by 60.17 and 7.96 g for males and females respectively. The growth rate during 0-4 weeks of age was 131.64, 130.68 and 132.35%, for SMGT, Bio and control respectively. In conclusion cGH Gene successfully isolate, molecular cloning from Cobb 500 and transferring by two methods SMGT and Bio to produce transgenic chickens of a local strain in Egypt .SMGT is an efficient method that will hopefully facilitate the imple-mentation of strategies for securing the benefits that can be expected to arise from the introduction of transgenic chicken, Bio open important new perspectives in the field of animal transgenic would be more rapid, with quick and effective delivery of genes to target tissues. Chicken cGH gene was effect in all growth traits and moved from the first generation to the second generation with the same shape and increased the effect. The second generation gave higher body weight at 0,4,8 and 12 weeks of age.