The physiological effect of black pepper and turmeric are of great interest due to their antioxidant, nutritional and therapeutic properties. Therefore, the target of this study was to investigate the physiological and immunological effects of black pepper to enhance bioavailability of turmeric on productive performance of growing Japanese quail. A total of two hundred unsexed seven day-old from Japanese quail were divided into four groups, each group was distributed into five replicates, 10 birds in each replicate. All groups were fed the same basal diet supplemented with 0.0%,2% turmeric powder/ Kg diet,0.5 % black pepper powder/ Kg diet for the 1^st , 2^nd and 3^rd groups, while the fourth group was given basal diet supplemented with a mixture of 2% turmeric powder + 0.5 % black pepper powder/ Kg diet. All quails were fed the experimental diets from 7 to 42 days of age. The results revealed that dietary supplements turmeric, black pepper either singly or in combination had significantly improved live body weight, weight gain and feed conversion ratio compared to control group. Additionally, results illustrated that combined black pepper + turmeric group had positive effect on protein fraction, globulin fractions(α, β, and γ-globulin), lipids profile, and immunoglobulin G (IgG) compared to other groups. Additionally, combined black pepper with turmeric group recorded highly significant values of thyroid hormones concentration, T3/T4 ratio and improvement SOD, MDA and GSH-Px concentration compared to other groups. Meanwhile, treated groups had no significant effect on feed consumption, liver functions markers (ALT, AST and ALP) and immunoglobulin A and M (IgA, IgM) compared to control group of growing quail. However, there were no significant differences between turmeric or black pepper groups on physiological and immunological responses of growing quails. Conclusion: These results concluded that combining of 0.5% black pepper to 2% turmeric together may enhance productive performance and had significantly improved serum lipids concentration, thyroid hormones, antioxidant enzymes activity, immunoglobulin G of growing quail.