A total of 192 unsexed day-old "Cobb" broiler chicks were used to evaluate the effects of different levels of dietary nano zinc oxide (N-ZnO) on nitrogen and minerals retention, blood serum measurements, meat chemical composition and tibia minerals of broiler chickens. Chicks were randomly distributed into six treatments of four replicates eight chicks each. A control contained 100 mg inorganic ZnO/kg diet (I-ZnO) and 100, 80, 60, 40 and 20 mg N-ZnO/kg diet were fed. Diets supplemented with different levels of N-ZnO had significant effect on blood serum parameters except for total protein and cholesterol. The highest contents of (albumin, high density lipoprotein, low-density lipoprotein, lactate dehydrogenase, superoxide dismutase, calcium, phosphorous and zinc) and the lowest (triglycerides, creatinine, uric acid, aspartate transferase, alanine transferase, alkaline phosphatase and malondialdehyde) were obtained with 20 mg N-ZnO/kg diet. A significant reduction in moisture and fat and significant increase in crude protein, ash and minerals (Ca, P and Zn) of breast and thigh meats were observed with N-ZnO treatments; the lowest and the highest percentages, respectively, of the former criteria were recorded with 20 mg N-ZnO. Birds fed N-ZnO (40 and 20), (60, 40 and 20) and 20 mg/kg diet had the highest significant tibia Ca, Zn and P percentages, respectively. Nano-ZnO treatments significantly increased the retention percentages except for Zn. Birds fed 40 mg N-ZnO/kg diet exhibited the highest nitrogen, Ca, P and Zn retention. It could be concluded that supplementation of N-ZnO to broiler diet improved birds' physiological status, meat carcass quality, bone mineralization and nutrients retention. The lower levels of N-ZnO (20 and 40 mg/kg diet) revealed promising results with no harmful effect on birds' health status.