Two trials were conducted to evaluate the efficiency of nano-zinc (ZnO-NPs) supplementations on hatchability, growth performance, carcass traits, bone characteristics and physiological status of Inshas chick.In the initial experiment, on day 18 of the incubation 1020 eggs in four groups (n=85, r=3) were injected with ZnO-NPs at levels of 0, 60, 80, and 100μg /egg.Results showed that in ovo injection with ZnO-NPs at different levels has no significant effect on the hatched chick weight. In addition, nano-zinc at the level up to 60µg/ egg has no adverse effect on hatchability percentage.In the second experiment 480 one-day-old unsexed chicks from the initial experiment with an initial weight of 33.64 ±0.98gwere fed on a diet containing nano-zinc at the level of 0 or 30 mg / kg diet. The interaction of ZnO-NPs pre/post-hatch supplementations showed a significant effect on final body weight, weight gain, feed intake, and feed conversion ratio and the non-treated chicks in the control group showed the lowest values. The highest spleen weightwas recorded at the level of 100 μg ZnO-NPs/egg. The highest content of zinc in the breast meat was observed in chicks supplemented with 100μg/egg and fed 30 mg/ kg diet. Tibia bone ash content increased with increasing nano-zinc in the diet, meanwhile tibia bone Zn and P contents were increased with increasing nano-zinc in ovo supplementation. Chicks fed 30 mg ZnO-NPs/ kg diet showed highest values for tibia length, thickness when pre-hatch eggs  supplemented with 100 μg ZnO-NPs/egg and the highest strength when pre-hatch supplemented with 60 μg ZnO-NPs/egg. ZnO-NPs in ovo supplementation had no significant effect on serum biochemical parameters. Total protein, albumin, ALT, AST, HDL, LDL, and serum Zn content values were increased with increasing nano-zinc in the diet except LDL.The highest phagocytic activities were observed in chicks fed 30 mg/ kg diet and pre-hatched with 60, 80, and 100 μg ZnO-NPs/egg.