The aim of this experiment was to evaluate the effects of spraying fertile eggs of Dandarawi chicken local strain with natural white vinegar solution (NWVS) on embryonic development, physiological parameters, hatchability, post-hatch chick growth and bacterial quantity on eggshell surface. Four hundred and fifty hatching eggs of Dandarawi chicken local strain were randomly distributed into five groups of 90 eggs each. Eggs of the 1^st group were served as a control (non–treated eggs). The 2^nd group was sprayed with water as a vehicle (positive control).  Eggs of the 3^rd, 4^th and 5^th groups were sprayed with three concentrations of vinegar: 1.25, 2.5 and 5%, respectively. Results showed that embryo weight, embryonic and shank lengths as well as, chick body weight, chick and shank lengths and hatchability tended to be Significantly higher (P<0.05) in eggs treated with natural white vinegar solution when compared with control eggs. While albumen weight ratio, egg shell thickness, egg weight loss ratio at 18^th day of development, embryonic mortality and hatch time were significantly (P<0.05) decreased in eggs sprayed with natural white vinegar solution when compared with control eggs. Blood constituents: RBCs, Hb and PCV%, plasma total protein, albumin, total lipids, P, Ca and T4, GH hormones were significantly increased (p<0.05), while plasma cholesterol and glucose were decreased. There was no effect on WBCs count compared to control. Internal organs of chicks at hatch and growth performance of chicks at 14^th days of age recorded significant higher values and improved feed conversion in response to spraying with natural white vinegar solution but yolk residual of chicks at hatch was less than control group. The use of natural white vinegar solution had significant influence on TBC and T. StaPhly. C.on egg shell surface either at one week or after two weeks of incubation compared to control groups. These results indicated that spraying fertile eggs of chicken (pre-incubation) with natural white vinegar solution as natural disinfectants is a good way to improve embryonic development, blood constituents, hormones, hatchability and performance of chicks.