The effect of green and brown seaweeds supplementation on laying performance and egg quality, serum lipid profile and antioxidant capacity was evaluated using laying Japanese quails. Five groups of Japanese quails (total n= 180, 120 laying quail hens + 60 male, 10 wks old), each in 3 replicates were used. Birds were fed either a basal diet (control) or basal diet supplemented with green (GS) and brown (BS) seaweeds each at 1.5 and 3% for 14 weeks. Results indicated that GS and BS supplementation can significantly improve egg-laying rate (P < 0.05) and increase egg number, egg weight and egg mass and improve feed conversion ratio (FCR) (P < 0.05). However, feed consumption (FC) was insignificantly affected by the different treatments. The GS and BS supplemented groups lead to improve the eggshell thickness, also helped to improve egg yolk weight, index and color compared to the control. Supplementing dried GS and BS significantly decreased the value of Haugh unit, significantly decreased the total lipids and total cholesterol content of egg yolk (P<0.05) as well as displayed higher fertility and hatchability percentages (P<0.05) than that of the control group. Quails which had received dried GS and BS treatments had a higher slaughter percentage and a lesser percentage of abdominal fat as compared to the control group. Serum total lipids, triglycerides, cholesterol, HDL and LDL values were significantly decreased as compared to the control group. There are significant effects (P<0.05) on enzymatic antioxidant activities of blood serum. It is concluded that green and brown seaweeds supplementation can improve egg-laying performance and had a positive effect on fertility and hatchability values and resulted in a significant reduction in total lipids and total cholesterol in the serum and yolk while enhancing total antioxidant capacity in blood serum as well as positively affecting economic parameters. Thus, these results suggested that green and brown seaweeds can be used safely as a non-feed additive in diets for laying Japanese quail.