The objective of this research, improving egg production and egg quality traits for Egyptian local breeds by detected loci genes using microsatellites (SSR) technique. Three Egyptian native breeds (Fayoumi, Dandarawi and Sinai) were reared under the same environmental, managerial and hygienic conditions from begin to hatching until end experimental (60 weeks of age). Egg weight, egg percentage, and broken percentage were recorded daily from the onset of lay within 3 months intervals. While, egg quality was evaluated at 36 weeks of age. The results appear that Fayoumi and Sinai hens were produced significantly highest egg production than Dandarawi ones. On the other hand, egg quality slightly differ among breeds on these study. The total number of alleles for seven primers and the diversity percentage within each breed. This data pointed that Dandarawi have the highest diversity while the Fayoumi breed have the highest similarity for the studied primers. On the other hand, when comparison among Fayoumi, Sinai and Dandarawi was carried out and indicated that, Dandarawi line was superior in difference of microsatellite markers compared Fayoumi and Sinai breeds. On the other hand, applied egg number (E.N) and egg production (E.P) traits for breeding programs reflected promising success in case of applying these traits on Fayoumi and Sinai breeds. The recommended that utilizing molecular markers for selection and therefore, be useful in breeding programs