In this study Jerusalem artichoke tubers powder (JA) as a source of prebiotics at 3% and 6% levels was supplemented in quail chicks, diet to determine the growth performance. An experiment of 42 days was conducted with a flock of 270 unsexed one- day old chicks, were used in a completely randomized design (CRD with 3 treatments and 3 replicates). Treatments were control no additive, T1containing 3% (JA), T2 containing 6% (JA). All chicks had free access to feed and water ad libitum during the 6-wk experiment. Weight gain (WG), feed intake (FI) and feed conversion ratio (FCR) were determined. At the end of experiment three birds per replicate were slaughtered. The results indicated that, chicks fed 3% and 6 % Jerusalem artichoke had higher body weight gain at 42 days and performance index also, lower feed intake; and better feed conversion ratio compared to the control group. Feeding diet containing 6% had the highest total serum protein, Glucose and AST concentrations; moreover were lower than in the control diet. No significant effect of different levels of Jerusalem artichoke on carcass characteristics was detected. Evidently, it may be concluded that the dietary supplementation of Japanese quail chicks with 6% Jerusalem artichoke resulted in considerable improvement in the growth and economic efficiency without adverse effects on carcass characteristics or constituents of blood serum.