The aim of this work was to study the efficacy of antibiotic growth promoter alternatives supplementation in the corn soybean meal diets on growth performance, carcass cuts and lymphoid organs %, some plasma parameters and some economic traits of broiler chicks. 150 one-day-old chicks (Arbor Acers), un-sexed were randomly divided into five experimental treatments with tree replicates per treatment group. The control group (T1) was fed basal (starter and grower) diets without supplementation, while other groups T2: T5 were fed basal diets supplemented with colistin antibiotic 1 g/ kg (Colistix®), Guanidinoacetic acid 0.6 g/ kg (Cre-Amino®), Lactobacillus acidophilus 1 g/ kg (Bio-Bac-Lac®) and Beta Mannanase 0.3 g/ kg (Hemicell®). At 32 days results revealed that, live body weight and daily weight gain for the broiler chicks fed T1, T2 and T3 diets were higher than those fed other dietary treatments. Chicks fed T2 and T3 diets had significant better feed conversion ratio compared with those fed T4 or T5 diets. The best protein (PCR), energy (ECR) conversion ratio and the highest performance index (PI) and production efficiency factor (PEF) were recorded for T1-T3 compared with other dietary treatments (T4: T5). Plasma cholesterol, HDL, LDL and GOT were not significantly different than control, while total lipids, triglycerides or GPT were significant. Difference in carcass cuts were not affected by treatments except breast % was significantly decreased than control. Chickens fed T3 diets showed the highest significant thymus ad bursa % compared with other dietary treatments. The results of economic traits showed that chickens fed basal diets supplemented with 0.6% guanidine acetic acid support and enhance economic efficiency without adverse effect on growth performance of broiler until 32 days of age. Therefore, it could be recommended from this study to