Two experiments were done to investigate in-ovo feeding of glucose (Exp. 1) for enhancing hatchability and hatching weight by using 300 fertile eggs of Fayoumi breeder. Eggs divided into 5 groups in 60 eggs/ treatment (20/ replicate). At 18^th day of incubation, eggs injected in the air sac as following groups:  first (control) without injection, second was punched (dry punch), third was injected by 0.1 ml distillated water (sham group), fourth and fifth were injected by 0.1 ml glucose at concentrationsof 2.5% and 5%, respectively. Results indicate that hatchability and hatching weight of fourth group recorded significant improvement by 14.71% and 5.68%, respectively compared with control. In the second experiment, a total number of 120-unsexed Fayoumi chicks, a day old, were arranged to 4treatments in 3 replicates (10 chicks each) as follows: the first group (control) was fed basal diet. The rest 3 groups were chosen from the former experiment, control without injection, groups injected with 0.1 ml glucose either at 2.5% and 5% concentration. All these 3 groups fed the basal diet incorporated with 5% glucose syrup from 1-28 days of age then switched to the control diet up to 56 days of age. Results show that the improvement in hatching weight was continued during starting and growing periods, also feed conversion ratio was enhanced by 10.23% for the group injected with 0.1 ml glucose 2.5% and fed diet contained glucose syrup compared to control. Carcass characteristics were not affected by any of the studied treatment except carcass % which significantly increased in chicks fed glucose syrup and injected with 0.1 ml glucose 2.5%. Conclusion: in-ovo feeding with glucose 2.5% improve hatchability and hatching weight of Fayoumi chicks. Moreover, incorporating hatched chicks with glucose syrup enhance performance without negative effect on blood glucose and lipid profile.