One hundred and twenty, 10-wk-old Gimmizah chickens, as a local balady strain were used in the current study to evaluate the physiological response to zinc (Zn) and/ or creatine monohydrate (CMH) supplementation in drinking water. All birds were weighed and randomly divided into four equal treatments. The first treatment fed the basal diet and served as control, while the second, third and fourth groups were given the basal diet and drinking water were supplemented with, 0.6 mg zinc sulphate/ml, 0.45 mg CMH /ml and 0.6 mg zinc sulphate /ml + 0.45 mg CMH /ml, respectively. Body weight gain, feed consumption, feed conversion, and relative weight of carcass, abdominal fat, and sex organs were calculated. Serum concentrations of total proteins, total lipids, triglycerides, total cholesterol, alkaline phosphates, thyroxin, testosterone andestradiol (E2) hormones were measured. The results indicated that the body weight gain, feed efficiency, and relative weight of carcass, abdominal fat, and sex organs were significantly (P≤0.05) increased in the fourth group as compared with the other treatments or the control groups. Moreover, plasma concentrations of total protein, total lipids, triglyceride, cholesterol, estradiol-17ß, testosterone, and thyroxin hormone levels in all supplemented groups were significantly (P≤0.05) higher than the control group. The highest values observed in the fourth group. Therefore, it could be recommended that supplementation of Zn + CMH might be involved in improving the physiological and productive performance of Gimmizah birds.