Two experiments were executed to study the impact of crude glycerin as an energy source in replacement of diet in Japanese quail on liver metabolism. In the first experiment, a total number of 180 newly hatched Japanese quail were used in a six-week study. The birds were randomly distributed into 4 experimental groups representing 4 dietary levels including crude glycerin 0% (control), 5%, 10% and 15% with three replicates 15 birds each. In the second experiment, a total number of 72 adult Japanese quail at 6 weeks of age were used in a ten week study. The birds were randomly distributed into 4 experimental groups (12 females and 6 males) representing the same dietary levels of crude glycerin used with the first experiment with three replicates 6 birds for each. In each experiment the liver fat percentage was significantly (p≤0.05) increased, while liver glycogen and hepatic cellular oxygen consumption decreased with increasing dietary glycerin levels. However, in the first experiment, male quail fed 15% glycerin showed higher creatine phosphokinase (CPK) levels compared to those fed other glycerin levels. Liver histological sections revealed fatty liver changes with increasing dietary glycerin levels. In conclusion, the results showed that the crude glycerin can be used as alternative energy source to replace (ex:corn) in growing and laying Japanese quail diets to the maximum level of 5% without adverse effects on liver metabolism.