This experiment aimed to study the effect of using distillers dried grains with soluble (DDGS) at different levels (0, 5, 10, 15%) treated with or without enzyme (avizyme1500) at level 0 and 1g/ kg diet on some immunological and physiological responses of broiler chicks. Two hundred and sixty five, Arbor acres unsexed broiler day-old chicks, were randomly divided into eight groups with 32 birds per each. The sequence of the eight dietary treatments were as follow: T1- Basal diet (without DDGS or enzyme); T2, T3 and T4- Basal diet contains 5, 10 and 15% DDGS without enzyme; T5, T6, T7 and T8- Basal diet contain 0, 5, 10, 15% DDGS with enzyme. The results showed that blood proteins for birds fed dietary DDGS at different levels significantly (p<0.01) increased than those fed control diet. The means of total lipids, triglyceride, cholesterol, AST and ALT in the broilers fed dietary DDGS (p<0.01) differed, while creatinine value was insignificantly affected. The means of AST values for broilers fed the diet containing enzyme significantly (p<0.01) decreased, while ALT level was not affected. Similarly, the means of T4 hormone concentration for broiler fed diet with adding enzyme significantly (p<0.01) reduced, while T3 insignificantly reduced compared with control diet. The mean PCV% (p<0.01) increased while, Hb reduced significantly (p<0.01) compared with control diet while, no significant differences were detected in RBC's and WBC's counts.
From the present results it can be concluded that using grade levels (5, 10 and 15%) of DDGS in broiler diets as untraditional ingredient (as a replacement of corn and soybean ingredients of diet) led to improve the physiological and immunological responses.