The object of this study was to evaluate the effect of graded levels of olive leaves extract (Oleuropein) supplementation on layer hen performance. One hundred and forty four of Bandarah chickens (24 wks) were randomly distributed to four treatment groups of three replicates (10 hens and 2 cocks per each replicate), group 1 was served as a control group. Group 2, 3 and 4 were fed diets supplemented with 50.0, 100.0 and 150 mg Oleuropein /kg diet, respectively. Birds were reared in floored cages in an open system house under similar conditions of management up to 42 wks of age. Results revealed that egg production, egg mass and feed conversion ratio were significantly (p<0.05) improved for the all groups supplied with different levels of oleuropein compared with control group. While egg weight and feed consumption were not affected by supplemental oleuropein. Egg yolk color score was significantly (p<0.01) increasing for the groups fed oleuropein diets. The significant highest egg yolk color score was recorded for the group fed diet supplied with the highest level of oleuropein (150 mg/kg diet). Egg yolk cholesterol significantly (p<0.05) decreased for oleuropein treatments. Significant decrease was observed in saturated fatty acids of egg yolk in treated groups (100, 150 mg oleuropein). Also, results showed significant increase in yolk omega 3 and 6 fatty acids for the groups supplied with oleuropein. Adding oleuropein to hens ‘diets at any tested levels resulted in significantly (p<0.01) increased in plasma protein and globulin. The same result was observed in plasma T3, while blood albumin, glucose concentrations and the activity of AST and ALT enzymes were not significantly differed among the all experimental groups. Blood lipid profile for the groups fed diets supplied with oleuropein was significantly (p<0.01) improved compared with the control group. In accordance with hematological parameters, addition of oleuropein at different levels significantly (p<0.01) increased white blood cells (WBC) and lymphocytes count while heterophils count and H/L ratio significantly (p<0.01) decreased. The improvement of superoxide dismutase (SOD) and total antioxidant capacity (TAC) were significantly (p<0.01) for the groups supplied with different levels of oleuropein compared with control group. Decrement of plasma lipid peroxidation based on MDA was significantly (p<0.001) in treated groups compared with control group. In conclusion, olive leaves extract (oleuropein) supplied up to 150 mg/kg diet improved the performance, lipid profile, immunological and anti-oxidative statues of Bandarah laying hens.