Chromosome homology is highly conserved between chicken (Gallus gallus) and Japanese quail (Coturnix japonica) and a few chromosome rearrangements occurred in the evolution of these two species. Blood samples were collected from chicken strains (21 Silver Montazah and 20 Mandara) and Japanese quail lines (22 Beige and 21 sample Grey). Eight chicken and six quail microsatellite markers were used reciprocally. Highly polymorphic microsatellite markers 12 out of 14 with ratio of 85.7% were successfully worked reciprocally and achieved PCR product in both species. The successful cross-species amplification led to identification of polymorphic markers that will be indispensable for identifying homologous regions on chromosomes. The majority of the loci were highly polymorphic information content (PIC) indicated that the most (12/14) of the selected microsatellite sequences could be serve the genetic diversity on different levels including conservation of such genetic resources, future improvements for these two poultry species and/or understanding different genome arrangement and knowledge interests.