: The aim of the study was to investigate the effect of supplemented chicken diets with 2% and 4% arginine (Arg) above NRC (1994) requirements on growth performance, immune response and gene expression of gene (y + LAT-2) in Fayoumi (Fa) and Golden Montazah (GM) chickens. In our previous study we fed Fa and GM layers the same arginine levels and we hatched their eggs to obtain their offspring. Offspring were sexed and sixty female within each strain per each level were selected and randomly divided into 4 replicate with 15 female chicks each. Each group within each strain fed the same dietary Arg level of their parents until 12 wks age. Body weight (BW), body weight gain (BWG), feed intake (FI) and feed conversion (FC) were recorded at 4, 8 and 12 wks of age. Antibody titers against Newcastle disease were measured at 5 days of age to estimate maternal immunity and at 12 wks of age to estimate acquired immunity. Immune organs weighed and their relative percent to live body weight was recorded.  Body weight was significantly increased as a result of Arg supplementation for all periods, while BWG was significantly increased during starter period. Neither FI nor FC were significantly affected by Arg treatments during the different periods. Relative liver weight was significantly decreased by Arg supplementation. Maternal and acquired immunity were numerically improved by Arg supplementation. Cationic amino acid transporter-1 mRNA in blood cells was greater in chicks fed 4% Arg than in chicks fed 2%. The Fa strain had higher gene expression of gene (y + LAT-2) than GM strain.