This study was performed to determine in vivo body composition of V-line doe rabbits through gestation and lactation by using Bioelectrical Impedance Analysis (BIA) technique. A total number of 45 V-line primiparous female rabbits aging 6-7 months were divided into three groups; 1st group: females were kept as pregnant (P, group), 2nd group: females were kept as lactating (L, group) and 3rd group: females were kept as lactating and pregnant in the same time (LP, group). Nine bucks (6 - 8 months age) were used for natural mating throughout the present study. The number of suckling kits was adjusted to 8 per doe, the kits were weaned at 30 days in L does group and 28 days in LP does group. Average feed intake (g) was weekly recorded. Body composition of does was determined by BIA technique using bioelectrical body composition analyzer (Quantum II) apparatus. Feed intake of P does group was increased (P