The aim of this study was to evaluate the influence of supplementing diet with propolis on Bandarah laying hens' performance. A total of 120 laying hens and 32 cocks at 30 weeks of age were randomly distributed among four treatments groups with 3 replicates each (10 females and 1 male/replicate) were housed in floor pens (2.5x1.5x2.5m).Birds in group 1 were fed a basal diet and considered as control group, while those in groups 2, 3 and 4 were fed on the same basal diet supplemented with 150,300 and 450 mg propolis/kg diet during experimental period (30-42 weeks of age). Results indicated that egg weight, egg production % and egg mass for propolis treatments were significantly (p<0.01) increased than those fed control diet. Feed consumption was not affected by supplemental propolis, while feed conversion ratio was significantly improved compared with control group. Shell thickness, Haugh unit score and egg yolk % significantly improved for hens fed diet supplemented with propolis compared with control group. However, egg shell %, shape index, yolk index, albumen % and yolk color score were not affected by propolis supplementation. In accordance with hematological parameters, addition of propolis at different levels significantly (p<0.01) increased Hb, PCV, RBC, WBC, lymphocytes count while heterophils count significantly (p<0.01) decreased. There was significant increase in plasma total protein, globulin, IgG and IgM with increasing propolis level. Lipid profile, Liver and kidney function significantly (p<0.01) improved for propolis treatments. Significant decrease was observed in plasma lipid peroxidation based on MDA levels in treated groups compared with control group also, results showed significant increase in antioxidants enzymes (TAC and SOD) for the groups supplied with propolis. Moreover, supplementation diets with propolis at different levels significantly improved semen quality, fertility and hatchability percentages compared with control. In conclusion, the results indicated that supplementation of propolis to Bandarah chickens diets significantly improved productive, reproductive, physiological, immunological and anti-oxidative status.