Purpose: This study was conducted to evaluate the effect of lavender oil on anxiety level in a group of Egyptian children. Subjects and Methods: A total number of 32 Egyptian children were included in this study. Children ages ranged from 4 to 7 years old in a good physical condition. Children were randomly distributed into two groups A & B each of 16 children. In children of experimental group B (using Lavender oil) each participant was instructed to inhale cotton with pure lavender oil for one minute before dental anesthesia. In control group (A) (children injected with dental anesthesia directly without lavender inhalation) facial image scale was used to evaluate anxiety level of children (FAS) and vital signs as pulse rate was measured using pulse oximeter for all children in both groups. Results: There was a significant reduction in anxiety level and pulse rate in experimental group. Conclusion: Lavender oil inhalation shows significant effect in reducing anxiety level and physiological parameters as pulse rate among children.