Purpose: This study was conducted to evaluate the effect of Garlic with lime mouth wash against Chlorhexidine mouthwash on salivary Streptococcus mutans count in a group of Egyptian children. Materials and Methods: A total of 40 Egyptian children were included in this study. Children ages ranged from (6 to 12) years old in a good physical condition. Children were randomly distributed into two groups A & B each of 20 children. In children of group A (using Garlic with lime) each participant was instructed to rinse with 5 ml of garlic with lime mouthwash for one minute. In group B (control group using Chlorhexidine mouthwash 0.1%) each participant was instructed to rinse with 5 ml of CHX for one minute. Results: There was a significant reduction in Streptococcus mutans count in both groups. Conclusion: garlic with lime mouthwash were successful as an antimicrobial agent. It significantly reduced the total bacterial count in the saliva of children when compared to a potent antiseptic like Chlorhexidine.